Associate Scientist with RNA, Oligonucleotide/siRNA/Immunoprecipitation/Western blotting/RT-qPCR, Mammalian/Cell Lines, and Molecular Biology

Associate Scientist with RNA, Oligonucleotide/siRNA/Immunoprecipitation/Western blotting/RT-qPCR, Mammalian/Cell Lines, and Molecular Biology Experience (Onsite Job in South San Francisco, CA)

Location: South San Francisco, CA

Terms: Contract W2 Only

Duration : 12 Months Contract (Will Get Extended for Long)

Description:

We are seeking a Research Associate to work on RNA post-transcriptional processing and regulation in human cells.

The Research Associate will perform experiments, maintain accurate records, and work closely with the project scientist to interpret results and determine the next steps.

The ideal candidate is curious, resourceful, and willing to ask questions, investigate unexpected results, and see experiments through to completion.

Preferred Qualifications

Bachelor’s degree in molecular biology, cell biology, biochemistry, genetics, or a related field, with at least 1-2 years of laboratory experience.

Experience with mammalian cell culture.

Experience with RNA isolation, RNA quality control, RNA sequencing library preparation, and NGS.

Experience with one or more of the following: oligonucleotide or siRNA perturbation, reporter assays, immunoprecipitation, Western blotting, or RT-qPCR.

Familiarity with RNA post-transcriptional processing such as splicing, translation or cleavage and polyadenylation.

Demonstrated ability to troubleshoot experiments, interpret results in the context of a research question, communicate clearly, and work both independently and collaboratively.

Daily functions

Maintain, passage, treat, transfect, and preserve mammalian cell lines. Monitor cell health and recognize potential issues with growth, media, or contamination.

Perform molecular biology experiments, including DNA and RNA purification, plasmid preparation, PCR, electrophoresis, and preparation of solutions and dilutions.

Conduct experiments, including reporter and minigene assays, RNA isolation, RT-qPCR, and preparation of next generation sequencing libraries. Perform additional RNA or protein assays as project evolves.

Skill Sets:

1. Research/Laboratory experience in molecular biology for at least 1 year, preferably more.

2. Familiarity with RNA biology, processing, and regulation (splicing, translation, cleavage, and polyadenylation)

3. Experience with one or more of the following: oligonucleotide or siRNA perturbation, reporter assays, immunoprecipitation, Western blotting, or RT-qPCR.

Preference to candidates who have experience in more or all.